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Anti-EphA2

From:abcam

Catalog Number : RS-0485R

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

Background: EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine （PTK） or serine/threonine （STK） kinase catalytic domains. The tyrosine kinase （TK） group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK （e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families）, and 32 of cytosolic TK （e.g. ABL, FAK, JAK, and SRC families）.
.Specificity : Anti- EphA2 is a Mouse monoclonal antibody              

specific for EphA2 of  Human,

–     use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–     Protein G affinity chromatography purification, purity :>95%

–     Isotype:  IgG

–     mol wt:  105kDa

Application :  

–      WB=1:100-500

–      ELISA=1:500-1000

–      IP=1:20-100 

–     IHC-P=1:100-500

–      IHC-F=1:100-500

–      IF=1:100-500

–      KLH conjugated synthetic peptide derived from human EphA2
optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.